IBCIT scientific output 2003-2008

Selected publications

1998

Grignani F, De Matteis S, Nervi C, Tomassoni L, Gelmetti V, Cioce M, Fanelli M, Ruthardt M, Ferrara,FF, Zamir I, Seiser C, Grignani F, Lazar MA, Minucci S and Pelicci PG. Fusion proteins of the retinoic acid receptor-α recruit histone deacetylase in promyelocytic leukemia. Nature. 391:815-818.

Nervi, C., Ferrara, F.F., Fanelli, M., Rippo, M.R., Tomassini, B., Ferrucci, P.F., Ruthardt, M., Gelmetti, V., Gambacorti-Passerini, C., Diverio, D., Grignani, F., Pelicci, P.G. and Testi, R. Caspases mediate retinoic acid induced degradation of the acute promyelocytic leukemia PML-RARa fusion protein. Blood. 92:2244-2251.

1999

Hansen L, Fjordvang H, Rasmussen SK, Vestergaard H, Echwald SM, Hansen T, Alessi D, Shenolikar S, Saltiel AR, Barbetti F, Pedersen O. Mutational analysis of the genes encoding protein kinase B α and β, phosphoinositide dependent protein kinase-1, phosphatase targeting to glycogen, protein phosphatase inhibitor-1, and glycogenin: lessons from a genetic study of the insulin stimulated glycogen synthesis pathway of skeletal muscle in NIDDM patients. Diabetes 48:403-407

2001

Ferrara FF, Fazi F, Bianchini A, Padula F, Gelmetti V, Minucci S, Mancini M, Pelicci PG, Lo Coco F, Nervi C. Histone deacetylase targeted treatment restores retinoic acid signaling and differentiation in acute myeloid leukemia. Cancer Res. 61, 2-7.

Njolstad PR, Sovik O, Cuesta-Munoz A, Bjorkhaug L, Massa O, Barbetti F, Undlien D, Shiota C, Magnuson MA, Molven A, Matschinsky FM, Bell GI. Neonatal diabetes mellitus due to complete glucokinase deficiency. N Engl J Med 344:1588-1592

2002

Bernassola F, Federici M, Corazzari M, Terrinoni A, Hribal ML, De Laurenzi V, Ranalli M, Massa O, Sesti G, McLean WHI, Citro G, Barbetti F, Melino G. Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient. FASEB J 16:1371-1378

Christesen HBT, Brock Jacobsen B, Odili S, Buettger C, Cuesta-Munoz A, Hansen T, Brusgaard K, Massa O, Magnuson MA, Shiota C, Matschinsky FM, Barbetti F. The second activating glucokinase mutation (A456V): implications for glucose homeostasis and diabetes therapy. Diabetes 51: 1240-1246

Di Croce L, Raker VA, Corsaro M, Fazi F, Fanelli M, Faretta M, Fuks F, Lo Coco F, Kouzarides T, Nervi C, Minucci S, Pelicci PG. Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor. Science 295, 1079-1082.

2003

Beissert T, Puccetti E, Bianchini A, Guller S, Boehrer S, Hoelzer D, Ottmann OG, Nervi C, Ruthardt M. Targeting of the N-terminal coiled coil oligomerization interface of BCR interferes with the transformation potential of BCR/ABL and increases sensitivity to STI571. Blood 102: 2985-2993

Cattelino A, Liebner S, Zanetti A, Gallini R, Balconi G, Corsi A,   Bianco P,   Wolburg H, Moore R,   Oreda B,   Kemler R and Dejana E: The conditional inactivation of beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. Journal of Cell Biology 162: 1111-1122

Cossu G, Bianco P: Mesoangioblasts. Vascular progenitors for extravascular mesodermal tissues. Currrent Opinions in Genetics and Development 13:537-542

Holmbeck K, Bianco P, Yamada S, Chrysovergis K Birkedal-Hansen H: MT1-MMP dependent, apoptotic remodeling of unmineralized cartilage: a critical process in skeletal growth. Journal of Cell Biology 163:661-671

Mangiacasale, R., Pittoggi, C., Sciamanna, I., Careddu, A., Mattei, E., Lorenzini, R., Travaglini, L., Landriscina, M., Barone, C., Nervi, C., Lavia, P., and Spadafora, C.: Exposure of normal and transformed cells to nevirapine, a reverse transcriptase inhibitor, reduces cell growth and promotes differentiation. Oncogene 22, 2750-2761

Riminucci M, Collins MT, Fedarko NS, Cherman N, Corsi A, White KE, Gupta A, Hannon T,MD, Econs MJ, Bianco P, Gehron Robey P: FGF-23 in fibrous dysplasia of bone and its relationship to renal phosphate wasting. Journal of Clinical Investigation, 112: 683-692

Sampaolesi M, Torrente Y, Innocenzi A, Tonlorenzi R, D'Antona G, Pellegrino MA, Barresi R, Bresolin N, De Angelis MG, Campbell KP, Bottinelli R, Cossu G. Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts. Science 301:487-92

2004

Cote S, McNamara S, Brambilla D, Bianchini A, Rizzo G, Del Rincon SV, Grignani F Nervi C, Miller WHJr. Expression of SMRT{beta} promotes ligand-induced activation of mutated and wild-type retinoid receptors.  Blood, 104: 4226-4235

Kuznetsov SA*, Riminucci M*, Ziran N, Tsutsui TW, Corsi A, Calvi L, Kronenberg HM, Schipani E, Gehron Robey P, Bianco P: The interplay of osteogenesis and hematopoiesis: expression of a constitutively active PTH/PTHrP receptor in osteogenic cells perturbs the establishment of hematopoiesis in bone and of skeletal stem cells in the bone marrow.
*Equal contributors J Cell Biol, 167: 1113-1122

2005

Colombo C, Delvecchio M, Zecchino C, Faienza MF, Cavallo L, Barbetti F and the Early Onset Diabetes Study Group of the Italian Society of Pediatric Endocrinology and Diabetology. Transient neonatal diabetes mellitus is associated with a recurrent (R201H) KCNJ11 (Kir6.2) mutation. Diabetologia 48:2439-2441

De Felice L, Tatarelli C, Mascolo MG, Gregorj C, Agostini F, Fiorini R, Gelmetti V, Pascale S, Padula F, Petrucci MT, Arcese W, Nervi C: 2005.  Histone deacetylase inhibitor valproic acid enhances the cytokine-induced expansion of human hematopoietic stem cells. Cancer Res, 65:1505-13

Fazi F, Rosa A, Fatica A, Gelmetti V, De Marchis ML, Nervi C, Bozzoni I. A mini-circuitry comprising microRNA-223 and transcription factors NFI-A and C/EBPα regulates human granulopoiesis. Cell 123, 819-31

Fazi F, Travaglini L, Carotti D, Palitti F, Diverio D, Alcalay M,  McNamara S, Miller WHJr, Lo Coco F, Pelicci PG, Nervi C. Retinoic acid targets DNA-methyltransferases and histone deacetylases during APL blast differentiation in vitro and in vivo. Oncogene 24, 1820-30

Foti D, Chiefari E, Fedele M, Iuliano R, Brunetti L, Paonessa F, Manfioletti G, Barbetti F, Brunetti A, Croce CM, Fusco A, Brunetti A. Lack of the architectural factor HMGA1 causes insulin resistance and diabetes in humans and mice. Nat Med 11:765-773

Gloyn Al, Odili S, Zelent D, Buettger C, Castleden HAJ, Steele AM, Stride A, Shiota C, Magnuson MA, Lorini R, d'Annunzio G, Stanley C, Kwagh J, van Schaftingen E, Veiga-da-Cunha M, Barbetti F, Dunten P, Han Y, Grimsby J, Taub R, Ellard S, Hattersley AT, Matschinsky FM. Insights into the structure and regulation of glucokinase from a novel mutation (V62M) which causes maturity-onset diabetes of the young. J Biol Chem 280:14105-14113

Insinga A, Monestiroli S, Ronzoni S, Gelmetti V, Marchesi F, Viale A, Altucci L, Nervi C, Minucci S. Pelicci, PG. Inhibitors of histone deacetylases induce tumor-selective apoptosis through activation of the death receptor pathway. Nat. Med., 11:71-76

Massa O, Iafusco D, D’Amato E, Gloyn AL, Hattersley AT, Pasquino B, Tonini G, Dammacco F, Zanette G, Meschi F, Porzio O, Bottazzo GF, Crinò A, Lorini R, Cerutti F, Vanelli M, Barbetti F and the Early Onset Diabetes Study Group of the Italian Society of Pediatric Endocrinology and Diabetology. KCNJ11 activating mutations in Italian patients with permanent neonatal diabetes. Hum Mutat 25:22-27

Meani N, Minardi S, Licciulli S, Gelmetti V, Lo Coco F, Nervi C, Pelicci PG, Muller H, Alcalay M “Molecular signature of Retinoic Acid treatment in Acute Promyleocytic Leukemia” Oncogene 24, 3358-68

Puccetti E, Zheng X, Brambilla D, Seshire A, Beissert T, Boehrer S, Nurnberger H, Hoelzer D, Ottmann OG, Nervi C, Ruthardt M. The Integrity of the Charged Pocket in the BTB/POZ Domain Is Essential for the Phenotype Induced by the Leukemia-Associated t (11;17) Fusion Protein PLZF/RAR alpha. Cancer Res 65:6080-88

Racanicchi S, Maccherani C, Liberatore C, Billi M, Gelmetti V, Panigada M, Rizzo G, Nervi C, Grignani F. Targeting fusion protein/corepressor contact restores differentiation response in leukemia cells. EMBO J. 24, 1232-42

Zardo G, Fazi F, Travaglini L, Nervi C. Dynamic and reversibility of heterochromatic gene silencing in human disease. Cell Res 15 (9): 679-690

2006

Benihoud K, Esselin S, Descamps D, Jullienne B, Salone B, Bobé P, Bonardelle D, Connault E, Opolon P, Saggio I, Perricaudet M. Respective roles of TNF-alpha and IL-6 in the immune response-elicited by adenovirus-mediated gene transfer in mice. Gene Ther. 2007 Mar;14(6):533-44

Cherubini G, Petouchoff T, Grossi M, Piersanti S, Cundari E, Saggio I. E1B55K-deleted adenovirus (ONYX-015) overrides G1/S and G2/M checkpoints and causes mitotic catastrophe and endoreduplication in p53-proficient normal cells. Cell Cycle;5(19):2244-52

Cimino,G, Lo-Coco,F, Fenu,S, Travaglini,L, Finolezzi,E, Mancini,M, Nanni,M, Careddu,A, Fazi,F, Padula,F, Fiorini,R, Aloe Spiriti,MA, Petti,MC, Venditti,A, Amadori,S, Mandelli,F, Pelicci,PG, and Nervi,C. Sequential Valproic acid/all-trans retinoic acid treatment reprograms differentiation in refractory and high-risk acute myeloid leukemia. Cancer Res. 66, 8903-8911

Galvez, B.G., Sampaolesi, M., Brunelli, S., Covarello, D., Gavina, M., Rossi, B., Costantin,  G., Torrente, Y. and Cossu G. Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migratory ability.  J. Cell Biol. 174: 231-43

Guccione E, Martinato F, Finocchiaro G, Luzi L, Tizzoni L, Dall' Olio V, Zardo G, Nervi C, Bernard L, Amati B. Myc-binding-site recognition in the human genome is determined by chromatin context. Nat Cell Biol. 8:764-770

Guttinger, M., Tafi, E., Battaglia, M., Coletta, M., Cossu, G.. Allogeneic mesoangioblasts give rise to alpha-sarcoglycan expressing fibers when transplanted into dystrophic mice. Exp Cell Res. Nov 15;312(19):3872-9

Merla,C., Liberti,M., Apollonio,F., Nervi,C., and D'Inzeo,G. Dielectric Spectroscopy of Blood Cells Suspensions: Study on Geometrical Structure of Biological Cells. Conf. Proc. IEEE Eng Med. Biol. Soc. 1, 3194-3197

Piersanti S, Sacchetti B, Funari A, Di Cesare S, Bonci D, Cherubini G, Peschle C, Riminucci M, Bianco P, Saggio I. Lentiviral transduction of human postnatal skeletal (stromal, mesenchymal) stem cells: in vivo transplantation and gene silencing. Calcif Tissue Int;78(6):372-84

Sampaolesi, M., Blot, S., D’Antona, G., Tonlorenzi, R., Innocenzi, A., Granger, N., Mognol, P., Galvez, B., Perani, P., Mantero, S., Guttinger, M., Pansarasa, O., Rinaldi, C., Cusella De Angelis, M.G., Torrente, Y., Bordignon, C., Bottinelli, R., Cossu, G. Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs. Nature. Nov 30;444(7119):574-9

Sentinelli F, Romeo S, Barbetti F, Berni A, Filippi E, Fanelli M, Fallarino M, Baroni MG. Search for genetic variants in the p66 shc longevity gene by PCR-single strand conformational polymorphism in patients with early-onset cardiovascular disease. BMC Genetics 7:14

Simomura K, Girard C, Proks P, Lippiat J, Cerutti F, Lorini R, Gloyn A, Hattersley AT, Barbetti F, Ashcroft FM. Mutations at the same residue (R50) of Kir6.2 (KCNJ11) that cause neonatal diabetes produce different functional effects. Diabetes 55:1705-1712

Barbetti F. Diagnosis of neonatal and infancy-onset diabetes. Endocr Dev 11:83-93

Benihoud K, Esselin S, Descamps D, Jullienne B, Salone B, Bobé P, Bonardelle D, Connault E, Opolon P, Saggio I, Perricaudet M.
Respective roles of TNF-alpha and IL-6 in the immune response-elicited by adenovirus-mediated gene transfer in mice. Gene Ther.;14(6):533-44

Dellavalle, A, Sampaolesi, M, Tonlorenzi, R,  Tagliafico, E,  Sacchetti, B,  Perani, L,, Innocenzi, B,  Galvez, BG,  Messina, G,   Morosetti, R,  Li, S,  Belicchi, M,  Peretti, G,  Chamberlain, JS,  Wright, WE,  Torrente, Y,   Ferrari, S,   Bianco, P and Cossu, G. Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. Nature Cell Biol. 9:255-67

DeMonte, L., Porcellini, S., Tafi, E., Sheridan, J., Gordon, J., Depreter, M., Blair, N., Panigada, M., Sanvito, F., Merati, B., Albientz, A., Barthlott, T., Ozmen, L., Blackburn, C., Guttinger, M. EVA regulates thymic stromal organization and early thymocyte development. Biochem Biophys Res Commun. May 4;356(2):334-40

Fazi, F., Racanicchi, S., Zardo, G., Starnes, L.M., Mancini, M., Travaglini, L., Diverio, D., Ammatuna, E., Cimino, G., Lo-Coco, F., Grignani,F. and Nervi, C. Epigenetic silencing of the myelopoiesis regulator microRNA-223 by the AML1/ETO oncoprotein. Cancer Cell 12: 457-466

Fazi, F., Zardo, G., Gelmetti, V., Travaglini, L., Ciolfi, A., Di Croce, L., Rosa, A., Bozzoni, I., Grignani, F., Lo-Coco, F., Pelicci, P.G., and Nervi, C. Heterochromatic gene repression of the retinoic acid pathway in acute myeloid leukemia. Blood 109, 4432-4440

Heegaard AM , Corsi A, Danielsen CC, Nielsen KL, Riminucci M, Young MF, Bianco P. Biglycan deficiency causes spontaneous aortic dissection and rupture in mice. Circulation 115: 2687-2688

Martina Y, Avitabile D, Piersanti S, Cherubini G, Saggio I., Different modulation of cellular transcription by adenovirus 5, DeltaE1/E3 adenovirus and helper-dependent vectors. Virus Res;130(1-2):71-84

Masia R, Koster JC, Tumini S, Chiarelli F, Colombo C, Nichols CG, Barbetti F. An ATP-binding mutation (G334D) in KCNJ11 is associated with a sulfonylurea-insensitive form of DEND (Developmental Delay, Epilepsy, and Neonatal Diabetes). Diabetes 56.328-336

Michienzi S, Cherman N, Holmbeck K, Funari A, Collins MT, Bianco P, Gehron Robey P, Riminucci M: GNAS transcripts in skeletal progenitors:  Evidence for random asymmetric allelic expression of Gsa. Human Molecular Genetics16:1921-1930

Mosig RA, Bowling O, DiFeo A, Ramirez MCM, Parker IC, Abe E, Diouri J, Al Aqeel A, Wylie JW,. Oblander SA, Madri J, Bianco P, Apte SS, Zaidi M, Doty SB,  Majeska RJ, Schaffler MB, Martignetti JA: Loss of MMP-2 disrupts skeletal and craniofacial development, and results in decreased bone mineralization, joint erosion and defects in osteoblast and osteoclast growth. Human Molecular Genetics, 16: 1113-1123

Nervi,C., Fazi,F., Rosa,A., Fatica, A. and Bozzoni, I. Emerging role for microRNAs in acute promyelocytic leukemia. Curr. Top. Microbiol. Immunol. 313, 73-84

Porzio O, Massa O, Cunsolo V, Colombo C, Malaponti M, Bertuzzi F, Hansen T , Johansen A, Pedersen O, Meschi F, Terrinoni A, Melino G, Federici M, Decarlo N, Menicagli M, Campani D, Marchetti P, Ferdaoussi M, Froguel P, Federici G, Vaxillaire M, Barbetti F. Missense Mutations in TGM2 Gene encoding transglutaminase 2 are associated with familial type 2 diabetes. Hum Mutat 28:1150

Sacchetti B, Funari A, Michienzi S, Di Cesare S, Piersanti S, Saggio I, Tagliafico E, Ferrari S, Gehron Robey P, Riminucci M, Bianco P: Self-renewing osteoprogenitors in bone marrow sinusoids can organize the hematopoietic microenvironment.  Cell 131: 324-336

2008

Baratta R, Rossetti P, Prudente s, Barbetti F, Sudano D, Nigro A, Farina MG, Trischitta V, Frittitta L. Role of the ENPP1 K121Q polymorphism on glucose homeostasis. Diabetes 57:3360-3364.

 
Barbetti F and the. Early onset diabetes Study Group of the Italian Society of Pediatric Endocrinology and Diabetes (SIEDP). Seven mutations in the human insulin gene linked to permanent neonatal/infancy onset diabetes mellitus. J Clin Invest 118:2148-2156

Beissert, T., Hundertmark, A., Kaburova, V., Travaglini, L., Mian, A.A., Nervi, C. and Ruthardt, M. Targeting of the N-terminal coiled coil oligomerization interface by a helix-2 peptide inhibits unmutated and imatinib-resistant bcr/abl. Int J Cancer 122:2744-52

Berghella, L., De Angelis, L., De Buysscher, T., Mortazavi, A., Biressi, S.,
Forcales, S.V.,  Sirabella, D., Cossu, G. and Wold, B. 2008. A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in post-natal muscle Genes & Dev. 22(15):2125-38.

Bianco P, Gehron Robey P, Simmons P: Mesenchymal stem cells: revisiting history, concepts and assays. CellStemCell 2:313-319

Bonfanti R, Colombo C, Nocerino V, Massa O, Iafusco D, Viscardi M, Chiumello G, Meschi F, Barbetti F. Insulin gene mutations as cause of diabetes in children negative for five type 1 diabetes autoantibodies. Diabetes Care. Published on line 2008 Oct 7, PMID 18840

Brufani C, Grossi A, Fintini D, Fiori R, Ubertini G, Colabianchi D, Ciampalini P, Tozzi A, Barbetti F, Cappa M. Cardiovascular fitness, insulin resistance and metabolic syndrome in severely obese prepubertal Italian children. Horm Res 70:349-356.

Caruso P, Burla R, Piersanti S, Cherubini G, Remoli C, Martina Y, Saggio I. Prion expression is activated by Adenovirus 5 infection and affects the adenoviral cycle in human cells. Virology In press.

Colombo C, Porzio O, Liu M, Massa O, Vasta M, Salardi S, Beccaria L, Liu M, Arvan P, Monciotti C, Toni S, Pedersen O, Hansen T, Federici L, Pesavento R, Cadario F, Federici G, Ghirri P, Iafusco D, Barbetti F and the. Early onset diabetes Study Group of the Italian Society of Pediatric Endocrinology and Diabetes (SIEDP). Seven mutations in the human insulin gene linked to permanent neonatal/infancy onset diabetes mellitus. J Clin Invest 118:2148-2156.

Fazi F and Nervi C. MicroRNA: Basic mechanisms and transcriptional regulatory networks for cell fate determination. Cardiovasc Res 79:553-61

Gargioli, C., Coletta, M., De Grandis, F., Cannata, S.M. and Cossu G.
PlGF-MMP9 expressing cells restore microcirculation and efficacy of cell therapy in old dystrophic muscle. Nature Med. 14(9):973-8.

Koster JC, Cadario F, Kurata HT, Peruzzi C, Colombo C, Nichols CG, Barbetti F. The G53D mutation in Kir6.2 (KCNJ11) is associated with neonatal diabetes and motor dysfunction in adulthood that is improved with sulfonylurea therapy. J Clin Endocrinol Metab 93:1054-1061

Leone, G., D'Alò F., Zardo, G., Voso, M.T. and Nervi, C. Epigenetic treatment of myelodysplastic syndromes and acute myeloid leukemias. Curr Med Chem 15: 1274-1287

Morey L., Brenner C., Fazi F., Villa R., Gutierrez A., Buschbeck M., Nervi C., Minucci S., Fuks F. and Di Croce L. MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks. Mol Cell Biol, 28:5912-23

Nervi, C., Fazi, F. and Grignani, F. Oncoproteins, heterochromatin silencing and microRNAs: a new link for leukemogenesis. Epigenetics 3, 1-4

Zardo, G., Cimino, G., Nervi, C. Epigenetic plasticity of chromatin in embryonic and hematopoietic stem/progenitor cells: therapeutic potential of cell reprogramming. Leukemia. 22:1503-18

2009

Brufani C, Ciampelini C, Grossi A, Fiori R, Fintini D, Tozzi A, Cappa M, Barbetti F. Glucose tolerance status in 510 children and adolescents attending an obesity clinic in Central Italy. Pediatr Diabetes, in press.

Brufani C, Tozzi A, Fintini D, Ciampalini P, Grossi A, Fiori R, Kiepe D, Manco M, Schiaffini R, Porzio O, Cappa M, Barbetti F. Sexual dimorphism of body composition and insulin sensitivity across pubertal development in obese Caucasian subjects. Eur J Endocrinol. Published on line 16 Feb 2009 PMID: 19221173.

Delvecchio M, Zecchino C, Faienza MF, Acquafredda A, Barbetti F, Cavallo L. Sulfonylurea treatment in a girl with neonatal diabetes (KCNJ11 R201H) and celiac disease: impact of low compliance to gluten free diet. Diab Res Clin Pract. Published on line 2 Apr 2009:doi:10.1016/j.diabres.2009.02.024.

Nocerino V, Colombo C, Bonfanti R, Iafusco D, Barbetti F. Mutations in IAPP and NEUROG33 genes are not a common cause of permanent neonatal/infancy/childhood onset diabetes. Diabetic Med, in press.

Travaglini L.,Vian L., Billi M., Grignani F. and Nervi C. Epigenetic reprogramming of breast cancer cells by valproic acid occurs regardless of estrogen receptor status Int J Biochem Cell Biol, 41:225-34